The Amazing Spider-Man (vol. 1) #22 (Mar. 1965):
“The Clown, and His Masters of Menace!”
by Stan Lee, Steve Ditko

The Amazing Spider-Man (vol. 1) #22

Title: “The Clown, and His Masters of Menace!”

Medium: comic

Cover date: Mar. 1965

Publisher: Marvel
Written by: Stan Lee
Art by: Steve Ditko

13 characters in this story:

(Click links for info about character
and his/her religious practice, affiliation, etc.)
Pub. #
Spider-Man Spider-Man (Peter Parker)
(lead character)
hero scientist
CBR Scale: I Protestant
Secret Defenders; Spider-Man and His Amazing Friends...  Marvel 10,664
Aunt May Aunt May supporting character
CBR Scale: S Protestant
[raised Peter Parker (Spider-Man)] Marvel 1,368
J. Jonah Jameson J. Jonah Jameson supporting character
CBR Scale: M hates Spider-Man
[Peter Parker's boss; Spider-Man's denouncer] Marvel 1,719
Venom Flash Thompson supporting character
CBR Scale: I Alcoholics Anonymous
U.S. Army Marvel 718
Betty Brant Betty Brant supporting character
CBR Scale: I Students of Love (temporary)
[former girlfriend of Peter Parker (Spider-Man)] Marvel 612
Liz Allan Liz Allan supporting character
  [longtime friend of Peter Parker] Marvel 429
Ringmaster Ringmaster (Maynard Tiboldt) villain
  Circus of Crime Marvel 106
The Great Gambonnos The Great Gambonnos (Ernesto and Luigi Gambonno) villain
  Circus of Crime Marvel 52
Human Cannonball Human Cannonball (Jack Pulver) villain
  Circus of Crime; Hood's Army Marvel 57
Princess Python Princess Python (Zelda DuBois) villain
  Circus of Crime; Femizons...  Marvel 93
Griffin Clown (Eliot Franklin) villain
  Circus of Crime; Gamma Corps...  Marvel 72
Mrs. Van der Twilliger supporting character
  [J. Jonah Jameson boasted of his art collection to her] Marvel 1
Giant-Man Giant-Man (Hank Pym)
(name seen in illustration (or on screen)) 
hero scientist
CBR Scale: U atheist
Commission on Superhuman Activities; Secret Defenders...  Marvel 1,543